Peer-reviewed publications

Publications featuring the use of EnPresso growth systems for the production of recombinant proteins. See also some examples of recombinant proteins expressed in EnPresso B growth systems as well as bacterial strains grown successfully.

Title Authors Institute Reference
Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli Jian Li et al. Technische Universität Berlin, Berlin, Germany Journal of Biotechnology, 2014 in press
Differential regulation of MAGE-A1 promoter activity by BORIS and Sp1, both interacting with the TATA binding protein Heidi Schwarzenbach et al. University Medical Center Hamburg-Eppendorf, Hamburg, Germany BMC Cancer 2014, 14:796
Apo, Zn2 {+}-bound and Mn2 {+}-bound structures reveal ligand binding properties of SitA from the pathogen Staphylococcus pseudintermedius Francesca Abate et al. Novartis Vaccines & Diagnostics, Siena, Italy Bioscience Reports (2014) Immediate Publication, doi:10.1042/BSR20140088
Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins Marco Becherelli et al. Novartis Vaccines and Diagnostics, Siena, Italy PLOS ONE, 2013, 8 (9):e74718
Identification of a novel zinc metalloprotease through a global analysis of clostridium difficile extracellular proteins Valeria Cafardi et al. Novartis Vaccines and Diagnostics, Siena, Italy PLOS ONE, 2013, 8 (11):  e81306
A fed-batch based cultivation mode in Escherichia coli results in improved specific activity of a novel chimeric – truncated form of tissue plasminogen activator F. Mahboudi et al. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Journal of Applied Microbiology 114, 364-372
Molecular basis for the catalytic inactivity of a naturally occurring near-null variant of human ALOX15 Thomas Horn et al. Charité, Berlin, Germany Biochimica et Biophysica Acta 1831 (2013) 1702–1713
Functional characterization of genetic enzyme variations in human lipoxygenases Thomas Horn et al. Charité, Berlin, Germany Redox Biology 1 (2013) 566–577
Functional characterisation of a metagenome derived family VIII esterase 4 with a deacetylation activity on b-lactam antibiotics Nobalanda Mokoena et al. CSIR Biosciences, Pretoria, South Africa Biochemical and Biophysical Research Communications, 437 (3): 342-348
High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli Kristof Zarschler et al. Institute of Radiopharmaceutical Cancer Research, Dresden, Germany Microbial Cell Factories 2013, 12:97
Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli Jennifer Jaitzig et al. Technische Universität Berlin, Germany ACS Synthetic Biology, 2013, doi: 10.1021/sb400082j
Effect of culture medium, host strain and oxygen
transfer on recombinant Fab antibody fragment
yield and leakage to medium in shaken E. coli
cultures.
Kaisa Ukkonen et al. BioSilta, Oulu, Finland Microbial Cell Factories 2013, 12:73