Examples of proteins/host strains

This information has been gathered from EnPresso users and peer-reviewed publications. See also Publications featuring the use of EnPresso growth systems for production of recombinant proteins.

Examples of recombinant proteins expressed in EnPresso B growth systems Bacterial strains grown successfully
Protein type Target protein Host strain
Protein domain CnaBC2, D5 and E3 domains – CnaB domains from Sdr family proteins (surface adhesins); SdrE proteins E. coli BL21 (DE3) – T1R
Proteases Extracellular metalloprotease of Clostridium difficile: 1.  Unknown hypothetical Zinc metallopeptidase CD630_28300: named Zmb1 and 2. FliD and 3. CD630_23650 E. coli T7 Express
Human proteases Novel chimeric-truncated form of tissue-type plasminogen activator (t-PA – a serine protease) with improved fibrin affinity E. coli BL21 (DE3)  with F– dcm ompT hsdS (rB– mB–) gal genotype
Human lipoxygenases Synonymous genetic variations in four different human lipoxygenase genes (ALOX5, ALOX12, ALOX15, and ALOX15B) E. coli BL21(DE3) pLysS
RNA-binding proteins Nucleolin
EF-hand domain-containing protein D2 (EFHD2)
Serine/arginine-rich splicing factor 1 (SRSF1)
Splicing factor U2AF (U2AF653)
E. coli BL21 (DE3) – T1R
Non-ribosomal peptide synthetases 654 kDa heterodimeric valinomycin synthetase (VlmSyn formed from Vlm1 and Vlm2) from Streptomyces tsusimaensis E. coli BL21 Gold
Single-domain antibodies (sdAbs) Single-domain antibodies (sdAbs) E. coli Shuffle® T7 Express
E. coli BL21(DE3)
Fab antibody fragments Fab antibody fragments E. coli RV308
E. coli BL21
Esterase Novel esterase (Est22) derived from an acidic leachate environment not specified
Cyclophilins Human cyclophilin-A E. coli C41[pEPPF:hCypA]
Dehalogenases Haloalkane dehalogenase: Spur
Haloalkane dehalogenase: LinB
Haloalkane dehalogenase: DbjA
Haloalkane dehalogenase: DhaA
E. coli BL21(DE3)
Dehydrogenases Lactobacillus alcohol dehydrogenase E. coli RB791 [F-, IN(rrnD-rrnE1), l-, lacIqL8]
Biotin-binding proteins Avidin Tes29 not specified
Biotin-binding proteins Bradavidin with C-terminal cysteine not specified
not specified not specified E. coli Rosetta DE3
not specified not specified E. coli BL21-A1