This information has been gathered from EnPresso users and peer-reviewed publications. See also Publications featuring the use of EnPresso growth systems for production of recombinant proteins.
Examples of recombinant proteins expressed in EnPresso B growth systems | Bacterial strains grown successfully | |
Protein type | Target protein | Host strain |
Protein domain | CnaBC2, D5 and E3 domains – CnaB domains from Sdr family proteins (surface adhesins); SdrE proteins | E. coli BL21 (DE3) – T1R |
Proteases | Extracellular metalloprotease of Clostridium difficile: 1. Unknown hypothetical Zinc metallopeptidase CD630_28300: named Zmb1 and 2. FliD and 3. CD630_23650 | E. coli T7 Express |
Human proteases | Novel chimeric-truncated form of tissue-type plasminogen activator (t-PA – a serine protease) with improved fibrin affinity | E. coli BL21 (DE3) with F– dcm ompT hsdS (rB– mB–) gal genotype |
Human lipoxygenases | Synonymous genetic variations in four different human lipoxygenase genes (ALOX5, ALOX12, ALOX15, and ALOX15B) | E. coli BL21(DE3) pLysS |
RNA-binding proteins | Nucleolin EF-hand domain-containing protein D2 (EFHD2) Serine/arginine-rich splicing factor 1 (SRSF1) Splicing factor U2AF (U2AF653) |
E. coli BL21 (DE3) – T1R |
Non-ribosomal peptide synthetases | 654 kDa heterodimeric valinomycin synthetase (VlmSyn formed from Vlm1 and Vlm2) from Streptomyces tsusimaensis | E. coli BL21 Gold |
Single-domain antibodies (sdAbs) | Single-domain antibodies (sdAbs) | E. coli Shuffle® T7 Express E. coli BL21(DE3) |
Fab antibody fragments | Fab antibody fragments | E. coli RV308 E. coli BL21 |
Esterase | Novel esterase (Est22) derived from an acidic leachate environment | not specified |
Cyclophilins | Human cyclophilin-A | E. coli C41[pEPPF:hCypA] |
Dehalogenases | Haloalkane dehalogenase: Spur Haloalkane dehalogenase: LinB Haloalkane dehalogenase: DbjA Haloalkane dehalogenase: DhaA |
E. coli BL21(DE3) |
Dehydrogenases | Lactobacillus alcohol dehydrogenase | E. coli RB791 [F-, IN(rrnD-rrnE1), l-, lacIqL8] |
Biotin-binding proteins | Avidin Tes29 | not specified |
Biotin-binding proteins | Bradavidin with C-terminal cysteine | not specified |
not specified | not specified | E. coli Rosetta DE3 |
not specified | not specified | E. coli BL21-A1 |