Proteins for NMR analysis

– more 15N labeled protein from bacterial cultures

Poor growth, low levels of expression and insufficient labeled protein are the most frequent challenges faced when using minimal medium to prepare proteins for structural characterization by NMR analysis.
EnPresso® B Defined Nitrogen-free is a pre-sterilized, chemically-defined growth system that increases the yield of 15N-labeled proteins.
Examples shown here are from independent laboratories – because results count.

64-fold increase in yield of 15N-labeled protein

Comparison with M9 growth medium
Result courtesy of Julia Smith, Senior Scientist and Dr. Ben Davis, Vernalis, UK

Results presented at ELRIG Advances in Recombinant Protein Technology. Download the scientific poster (login required): Improvements in yield of 15N-labeled proteins