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EnBase Opti-Set 2 x 24 deep-well plate set

EnBase™ OptiSet is a handy tool which helps you to select an optimal cultivation medium for the production of your recombinant protein.
It includes a total of 8 different conditions for testing. One of the test media is the medium which you routinely use for recombinant protein production. The other medium/cultivation options utilize the patented EnBase technology of BioSilta.
EnBase™ enables controlled growth of bacteria by enzymatic glucose feeding.
This optimisation procedure is very simple and requires low hands-on time.
Investing 2 hours with this OptiSet product will help you to
a) find a cultivation method which is optimal for the type of protein which you
are expressing
b) find a method which yields a substantial (ten-fold) increase in bacterial cell number and product yield
c) find a method which can be easily scaled up to larger volumes
d) find a method which allows you to reduce the volume of your cultures
Additionally, the use of EnBase™ technology allows you to use higher induction cell densities and longer induction times than those which are typically used in shake flask cultivations. By using recommended cultivation procedures, you will obtain increased flexibility, and the growth of bacteria will follow your schedule and expectations. Controlled slow growth of bacteria will bring you a new level of control. In finding an optimal way of cultivating bacteria and producing recombinant proteins, you can save several days of work by having a reliable high yield cultivation method in your hands.

Optimisation set components and storage conditions

Item	Contents	Storage
1. EnBase™ 24 deep-well Plate	1 plate	RT, 5 weeks
1a. Oxygen permeable membrane covers	4 pcs. for cultivation	RT
2. EnZ I’m (3000 U/L):	200 µL, sterile	+4 °C, 3 months
3. EnBase™ Medium for dilution	20 ml, sterile	RT, 5 weeks
4a. Medium supplement: Thiamine	100 µl, sterile	RT, 3 months
4b. Medium supplement: Magnesium	200 µl, sterile	RT, 3 months
5. Medium Booster	900 µl, sterile	RT, 5 weeks
6. Medium Booster	3.18 ml, sterile	RT, 5 weeks

Description of other vials

Item	Contents	Storage
7. EnBase™ Mineral Salt Medium (MSM 2)	20 ml, sterile	RT, 5 weeks
8. EnBase™ Balanced Medium (MSM 10)	20 ml, sterile	RT, 5 weeks
9. EnBase™ Flo Medium	20 ml, sterile	RT, 5 weeks
*10.* *Empty vial for standard medium*	Sterile, use for your own standard medium
Vials for harvesting the cells	24 pcs. of 2 ml coloured tubes, non-sterile.	RT

Storage of the set components: See the best before-date and storage conditions from the packages. RT: Room temperature, 18-25 °C

2. EnZ I’m is stable at room temperature but should be stored at + 4 °C on arrival to avoid contamination.

EnBase™ Media: After addition of Thiamine (4.), use fresh.

Disposal of spare media: The medium components are not harmful in small concentrations and can be discarded according to local rules without precautions.