We currently have two EnBase products available, the 96 well standard plate and the 1 litre shake flask product.


Please see below for comparison and prices.

96 well standard plate product

• EB96S06 EnBase 6 plate Cultivation Set, (60 well capacity per plate) €320
  
• EB96S12 EnBase 12 plate Cultivation Set, (60 well capacity per plate) €625

96 well standard plate product specification:

• Glucose limited fed-batch EnBase technology. Glucose is released enzymatically from the polymer matrix that is attached on the bottom of the wells.
  
• Maximum cultivation volume is 150 µl. Original well volume is 400 µl, of which the gel takes 150 µl.
  
• 60 Cultivations per plate, outermost wells of 96-well plate are used for humidification of the plate.
  
• Applicable strains: W3110, RB791, RV308, BL21 and its DE3 derivatives, Rosetta.
  
• Not recommended: Strain specific genetic markers: amino acid auxothrophy, relA and spot mutants, redox mutants.
  
• Minimal mineral salt medium MSM-medium recommended to obtain glucose fed-batch type process. Addition of complex compounds is possible, but MSM-medium is recommended to be used as basis to obtain high cell densities
  
• Humidity: 60 % of relative air humidity in the cultivation chamber to avoid precipitation.
  
• Shaker suitable for plate cultivations has a small amplitude and high speed. The speed shall be adjusted to avoid culture transfer to the plate closure.
  
• Use plates within 5 weeks of purchase, Store as instructed within user manual.
  
  

Cultivation Set Contains the following
Elements:

6 (12) pieces of Sterile EnBase 96 microwell plates, 1(2) Vial MSM Medium Sterile, 1 Vial Sterile EnBase enzyme 300 units/ml, Sterile Vial
for enzyme dilution, User manual, package insert.