How EnBase Works
How does EnBase grow healthier and more productive microorganisms?
EnBase promotes the growth of healthier and more productive cells by slowly releasing the glucose from a polysaccharide. This degradation is enzymatically-controlled and releases glucose in a slow and desired manner; thus controlling the growth rate of the microorganism.
The ability to control the bacteria growth allows the researcher to optimise the culture conditions based on his/her needs. The slower growth rate prevents the issue of overfed microorganisms, excessively fast growth of the culture and the resulting oxygen depletion.
Since the bacteria are growing slowly and the glucose concentration in the medium is minimal, the cultures using EnBase media are capable of progressing for over 48 hours while maintaining optimal pH levels.
Our protein expression protocols suggest an overnight culture (~16 h) prior to the induction period, which can continue for 24 hours. This extended culture and induction time allows the microorganism to grow slowly producing more cell mass and an increased amount of soluble, active protein. The success of the EnBase media is due to the slow growth to prevent the typical detrimental conditions that lead to poor quality results, such as low pH, low O2 or side metabolites.
BioSilta scientists have scaled-down fed-batch technology to the volume of a microwell.
