Don’t wait for a new tradition Just use EnBase! A customer account

September 18, 2009

I have used EnBase Flo in my recombinant protein expression experiments and I have compared the results to my former system (M9ZB medium). I can easily give you the following statement:

Time is a precious commodity in protein structural and functional research. To obtain enough material for protein purification and crystallization by E. coli cultivations, have had to use large culture volumes and many repeats. These cultivations have often failed to produce adequate product quantity and quality. My proteins are large in size, which makes their production in E. coli a very challenging task. Cultivation, cell harvesting and protein purification activities often take much more time than my real interests: protein structural and functional analysis. This was very frustrating, but now I realize that I was stuck with tradition. Having been introduced to EnBase from BioSilta my research has gained new energy. One cultivation in 100 ml volume provides me more protein in a soluble (correctly folded) format than 2 liters cultivations previously, and I don’t need to repeat these cultivations! Don’t wait for a new tradition, just use EnBase!
Tatu Haataja Dept. of Biochemistry University of Oulu